Drug Research
Drug Discovery & Development

The Electrospinning Company - Polymer Scaffolds for 3D Cell Culture Case Studies

The Electrospinning Company - Polymer Scaffolds for 3D Cell Culture

Mimetix Scaffolds for 3D Cell Culture and Tissue Engineering Electrospun scaffolds are highly porous and mimic the extracellular matrix, providing an ideal environment to support the growth of cells in 3D. They are created by electrospinning polymers into nanofibres or microfibres. Xeno-free, FDA-approved, medical grade polymers can be selected to facilitate translation of research findings into clinical use.
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Mimetix Scaffolds for 3D Cell Culture and Drug Discovery Cells grown in a 3D micro-environment look and behave more like cells in human tissues than those cultured in 2D. They are in contact with other cells and produce extracellular matrix, which influences tissue-specific gene expression, cell growth and the uptake and metabolism of drugs. 3D cell-based in vitro assays can provide more realistic data on the efficacy of drug candidates against tumour cells or their toxicity, potentially reducing the risk of failure in expensive clinical trials.
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Mimetix® Electrospun Scaffolds for 3D Culture of Upcyte® and Primary Hepatocytes This study investigates the Mimetix® electrospun scaffold, which consists of highly uniform electrospun PLLA microfibres supporting the three-dimensional growth of cells, as a cell-based assay system for upcyte® and primary hepatocytes.
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Mimetix for 3D Culture Human Epithelial Breast Cancer Cells The objective of this study was to test if the Mimetix® electrospun scaffold is a suitable tool for the culture of human epithelial breast cancer cells in 3D, which can be used in drug discovery programmes for in vitro toxicity and efficacy assays. Cell viability and growth in 12-well plates and 96-well plates was shown to be highly consistent (cv < 10%) and cells were shown to be more resistant to drug-induced apoptosis following 10 days of proliferation in the Mimetix scaffolds than on 2D tissue culture plastic, indicating that they had assumed a 3D phenotype.
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