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Tailor Made Clonal Cell Lines

By SIRION BIOTECH

Custom cell lines created and modified with an expression vector of your choice with optimal transgene expression, guaranteed stability, reliable cell banking and ready-to-use in just 4-6 weeks.

Service Comprises

  • Transfection/transduction of Your Cell Line with an expression vector of your choice
  • Generation of stable cell pools by antibiotic selection.
  • Establishment of single cell clones using the Genetix Clone Select Imager
  • Small scale expansion of individual clones
  • Identification of "High Performers" via quantification of target gene or GOI expression by qRT-PCR and/or Western Blot relative to control cells
  • Large scale expansion and cell banking of selected clones
  • Final QC: Transgene expression, growth characteristics, long-term cultivation (20 passages) optional: marker gene expression profiling, genotyping, genomic mapping of transgene integration site

Benefits

  • Clonal cell lines with optimal transgene expression
  • Guaranteed stability of cell clones
  • Cell banking of individual cell clones for high inter assay stability
  • Frozen cells ready-to-use

Available service

  • Clonal Gene Knockdown cell lines
  • Clonal Gene Overexpression cell lines
  • Clonal Reporter Gene cell lines (e.g. luciferase, fluorescent reporter)
  • Clonal FRT cell lines for isogenic transgene expression
  • Clonal cell lines for TET ON/TET-OFF inducible gene expression