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1.7 Quantifying Amplicons in Non-Purified PCR Reactions: Direct Assessment of Product Yield

Published 13 April 2016 | By Trinean

The polymerase chain reaction (PCR) is a fundamental tool in molecular biology and clinical testing. Most genetic tests rely upon the power of PCR to enrich specific target-sequences prior to analysis. Analytical methods like sequencing are relatively expensive and labs want to minimize the resources spent on samples that don't meet the requirements of concentration and quality to deliver a reliable result.

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1.7 Quantifying Amplicons in Non-Purified PCR Reactions: Direct Assessment of Product Yield