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RNA Isolation

By vertis Biotechnologie

VERTIS are experts in the isolation of high-quality RNA from valuable and challenging starting materials.

Isolation of all types of RNA

  • Isolation of total RNA from a wide variety of starting materials (animals, plants, algae, fungi, protists, bacteria)
  • Isolation of small non-coding RNA, especially miRNA
  • RNA isolation from mixed samples (e.g. infected tissues)
  • RNA isolation from environmental samples (e.g. stuhl, sea water, glacial ice, etc.)
  • Isolation of total RNA from FFPE samples
  • Each RNA preparation will be quality checked on a Shimadzu MultiNA microchip electrophoresis system any by UV/Vis spectroscopy

Enrichment strategies for specific RNA species

We offer different strategies for the removal of ribosomal RNA (rRNA) molecules from total RNA preparations or for the enrichment of full-length mRNA or of small RNA species.

Eukaryotic RNA

RNA species

Enrichment strategy

Poly(A)+Oligo(dT) chromatography
Full-length mRNACAP selection (enzymatic or antibody)
Small non-coding RNA (sncRNA)Selective elution from silica gel (< 200nt)
MicroRNASize fractionation (15 -30nt) with an automated fractionation system
rRNA depleted total RNAEnzymatic degradation of rRNA or removal or rRNA molecules with subtractive hybridization

Prokaryotic RNA

RNA species

Enrichment strategy

5'PPP primary transcriptsEnzymatic selection of 5'PPP ends
Small non-coding RNA (sncRNA)
Selective elution from silica gel (< 200nt)
rRNA depleted total RNAEnzymatic degradation of rRNA or removal or rRNA molecules with subtractive hybridisation

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RNA-Analysis with a Shimadzu MultiNA microchip electrophoresis system

Click here for more information.